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STEMLight™-96 Research

A Flexible Absorbance Proliferation, Viability and Functional Assay for Multiple Cell Types.

Uses of STEMLight™-96 Research
An alternative to STEMFluor™-96 Research and STEMGlo™-96 Research using an absorbance plate reader.
A proliferation, viability, cell functionality and cell number assays for numerous cell types derived from multiple organs and tissues.
Developmental biology.
Determine self-renewal and expansion ability and potential.
Studies on the cell proliferation process.
Determination of cells into differentiated cells.
Cell-based therapies.
Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) cells and cell lines.
Comparison of ES- or iPS-derived stem cell response with primary stem cells.
Benefits of using STEMLight™-96 Research
Uses a absorbance plate reader or multiparameter plate reader, available in most laboratories.
Non-subjective, instrument-based and quantitative readout procedure.
Available for adherent or non-adherent cells.
Multiplexes with flow cytometric assays.
Multiplexes with gene expression assays etc for more information from a single sample.
Flexibility to use virtually any in vitro stem cell protocol, reagents and growth factors/cytokines.
High throughput capability.
Fast to learn and easy to use.
Species
Multiple
Cell Sources
STEMGlo™-96 Research can be used to study virtually any cell type with proliferation capability or high intracellular ATP concentrations. These include, but are not limited to cells from the following organs and tissues:

Bladder
Brain
Breast
Eye
Gut
Heart
Kidney
Lung
Liver
Ovary
Prostate
Skin
Embryonic tissue
Fetal tissue.
Other cell types that can be studies using STEMGlo™-96 Research include:

Proliferating cell lines
Tumor cells
Embryonic stem (ES) cells
Induced puripotent stem (iPS) cells
ES-or iPS-derived cells
STEMLight™-96 Principle
Incorporates a customized Promega CellTiter 96® AQueous One reagent that measures the amount of soluble formazan by absorbance at 490nm produced by a MTS tetrazolium reduction, colorimetric reaction.
After culture, just add 20μl of the MTS reagent, mix and read absorbance at 490nm after 1-4 hours in a 96-well plate reader. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
No solubilization step as in a MTT reaction. Replaces MTT assays.
Read results between 1-4 hours after addition of MTS reagent. Plates can be returned to incubator and re-read at a later time for increased sensitivity.
Use
For Research Use Only. Not for clinical diagnostic use.

Major Equipment Required
Absorbance plate reader or multiparameter plate reader with a 490nm filter.

Kit Contents
MTS Reagent
Sterile, 96-well plates
Non-sterile, 96-well plate for background determination
Sterile, adhesive foil covers to maintain sterility of unused wells
Instruction manual


Related Assays
STEMGlo™
STEMFluor™
STEMGlo™-PREP: to detect self-renewal and expansion potential of primary stem cells and stem cell lines
HALO®-96 Research: bioluminomics™ assays for human and animal lympho-hematopoietic stem cells
HALO®-96 PREP: to detect self-renewal and expansion potential of lympho-hematopoietic stem cells
HemoFLUOR™-96: fluorescence assays for lympho-hematopoietic stem cells
HemoLIGHT™-96: absorbance assays for lympho-hematopoietic stem cells
MSCGlo™-96: bioluminomics™ assays for mesenchymal stem cells
MSCFluor™-96: fluorescence assays for mesenchymal stem cells
MSCLight™-96: absorbance assays for mesenchymal stem cells


Resource Information

YouTube Videos
Visit our Technical Guide Video Page

Brochures
2014 Cellular Therapy & Regnerative Medicine Brochure

Background
Proliferation & Differentiation
Assay Readouts
Assay Standardization

Bioluminescence Assays
STEMGlo™-96 Research
STEMGlo™-96 PREP
STEMClone™
STEMGlo™-Tox HT
STEMGlo™-96 PRT
NeuroGlo™-Tox HT
HepatoGlo™-Tox HT

Fluorescence Assays
STEMFluor™-96 Research

Absorbance Assays
STEMLight™-96 Research

Contract Services
Toxicity to Primary Cell Systems and Cell Lines
Hepatotoxicity Testing
Neurotoxicity Testing
Drug-Drug Interaction
Comparative Toxicity Testing

Principais Aplicações
Stem Cells Reseacrhing

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