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STEMFluor™-96 Research

A Flexible Fluorescence Proliferation, Viability and Functionality Assay for Multiple Cell Types .
Uses of STEMFluor™-96 Research
An alternative to STEMGlo™-96 Research if a luminescence plate reader is not available.
A proliferation, viability, functionality and cell number assay for for numerous cells types including primary stem cells, cell lines and primary explanted cells.
Developmental biology.
Determine self-renewal and expansion ability and potential.
Studies on the cell proliferation process.
Determination of cells into differentiated cells.
Cell-based therapies.
Determine the proliferation/differentiation response/effects of growth factors/cytokines media and other factors on primary (adult) stem cells and stem cell lines.
Comparison of ES- or iPS-derived stem cell response with primary stem cells.
Benefits of using STEMFluor™-96 Research
Uses a fluorescence plate reader or multiparameter plate reader with an excitation filter at 390-400nm and an emission filter at 505nm.
Non-subjective, instrument-based and quantitative readout procedure.
Available for adherent or non-adherent cells.
Multiplexes with flow cytometry allowing cells to be detected using other fluorescent labels/markers.
Multiplexes with luminescence assays.
Multiplexes with gene expression assays etc for more information from a single sample.
Flexibility to use virtually any in vitro cell protocol, reagents and growth factors/cytokines.
High throughput capability.
Fast to learn and easy to use.
Species
Multiple
Cell Sources
STEMGlo™-96 Research can be used to study virtually any cell type with proliferation capability or high intracellular ATP concentrations. These include, but are not limited to cells from the following organs and tissues:
Bladder
Brain
Breast
Eye
Gut
Heart
Kidney
Lung
Liver
Ovary
Prostate
Skin
Embryonic tissue
Fetal tissue.
Other cell types that can be studies using STEMGlo™-96 Research include:
Proliferating cell lines
Tumor cells
Embryonic stem (ES) cells
Induced puripotent stem (iPS) cells
ES-or iPS-derived cells
STEMFluor™-96 Principle
Incorporates a customized Promega CellTiter Fluor™ reagent that detects protease activity only in living cells. Fluorescence produced only when the GF-AFC substrate is cleaved by protease activity to produce a fluorescence signal proportional to the number of living cells.
After culture, just add 100μl of the reagent, mix and measure signal between 30 minutes and 3 hours in a plate reader. Optimal incubation time: 2 hours.
Uses a plate fluorometer or multiparameter plate reader to measure fluorescence with an excitation filter of 380-400nm and an emission filter of 505nm.
Use
For Research Use Only. Not for clinical diagnostic use.
Major Equipment Required
Fluorescence plate reader or multiparameter plate reader.

Kit Contents
GF-AFC Reagent
Sterile, 96-well plates, depending on assay use
Sterile, adhesive foil covers to maintain sterility of unused wells
Instruction manual


Related Assays
STEMGlo™ HT
STEMLight™
STEMGlo™-PREP: to detect self-renewal and expansion potential of primary stem cells and stem cell lines
HALO®-96 Research: bioluminomics™ assays for human and animal lympho-hematopoietic stem cells
HALO®-96 PREP: to detect self-renewal and expansion potential of lympho-hematopoietic stem cells
HemoFLUOR™-96: fluorescence assays for lympho-hematopoietic stem cells
HemoLIGHT™-96: absorbance assays for lympho-hematopoietic stem cells
MSCGlo™-96: bioluminomics™ assays for mesenchymal stem cells
MSCFluor™-96: fluorescence assays for mesenchymal stem cells
MSCLight™-96: absorbance assays for mesenchymal stem cells

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