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HALO®-Tox HT Predictive Hemotoxicity Platform

Predictive In Vitro Stem and Progenitor Cell Hemotoxicity Screening and Testing Platform for all Stages of Drug Development and Xenobiotics.
Uses of HALO®-Tox HT
Predictive in vitro to in vivo surrogate assay for human hemotoxicity.
Predictive in vitro to in vivo surrogate assay for all animal hemotoxicity testing.
Predictive in vitro stem and progenitor hemotoxicity screening and testing platform for all stages of drug development, including patient monitoring during clinical trials.
Predictive in vitro stem and progenitor hemotoxicity screening and testing for xenobiotic agents.
Incorporation into routine high throughput ADME/Tox screening.
Drug / compound ranking according to species and/or cell population sensitivity.

Benefits of using HALO®-Tox HT for Hemotoxicity Testing
A 3Rs Alternative Assay Platform for Reduction, Refinement and Replacement of animal testing.
The only in vitro high throughput stem and progenitor hemotoxicity assay platform available with both 96-well and 384-well plate formats (HALO®-96 Tox and HALO®-384 HT).
Highly predictive with greater than 80% concordance between in vitro and in vivo results (Toxicol Lett. 2009; 188:98-103).
Rapid turnaround: results in 5 days for human and non-human primate cells and 4 days for other animal cells.
Validated against the Registry of Cytotoxicity Prediction Model as a cytotoxicity assay.
Estimate in vivo starting doses and maximum tolerated doses (MTD) from in vitro IC values.
Incorporates Suspension Expansion Culture and proven Bioluminomicsâ„¢ Technology for the most advanced and reliable hemotoxicity assays available.
Designed for multiplexing with other assays to produce the most amount of information from a single sample.
Species
Human
Non-human primate (Cynomologus and Rhesus)
Dog
Rat
Mouse
Horse, pig and sheep also available as custom orders.
Tissues
Bone marrow; human and animal tissues
Peripheral blood; human and animal tissues
Umbilical cord blood; human and some animals
Spleen; rodents
Fetal and adult liver; rodents
Yolk sac; rodents

Cell Populations Detected using HALO®-Tox HT
Stem Cells
• HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L
• HPP-SP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L
• CFC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
• CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
• CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
• CFC-GEM 2: EPO, GM-CSF, IL-3, SCF
• CFC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

Progenitor Cells
• BFU-E 1: EPO, IL-3, SCF
• BFU-E 2: EPO
• GM-CFC 1: GM-CSF, IL-3, SCF
• GM-CFC 2: GM-CSF, G-CSF, IL-3, SCF
• GM-CFC 3: GM-CSF
• Mk-CFC 1: TPO, IL-3, SCF
• Mk-CFC 2: TPO

Lymphopoietic cells
• T-CFC: IL-2
• B-CFC: IL-7

No growth factors: Add growth factors/cytokines of choice.
Cell Populations for "Global" Hemotoxicity Testing
4 Population Hemotoxicity Assay Kits (4 Pop)

CFC-GEMM 1
BFU-E 1
GM-CFC 1
Mk-CFC 1
+ Background control
5 Population Hemotoxicity Assay Kits (5 Pop)

HPP-SP 2
CFC-GEMM 1
BFU-E 1
GM-CFC 1
Mk-CFC 1
+ Background control
7 Population Lympho-Hemotoxicity Assay Kits (7 Pop)

HPP-SP 2
CFC-GEMM 1
BFU-E 1
GM-CFC 1
Mk-CFC 1
T-CFC
B-CFC
+ Background control

Examples of HALO®-Tox HT
Please go to our Contract Services: Hemotoxicity and Blood-Forming System Studies to see examples and other information on using HALO® for in-house hemotoxicity studies

Use
For Research Use Only.

Major Equipment Required
A plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

Kit Contents
HALO®-Tox HT Master Mix(s)
ATP standard
ATP high and low controls
ATP Enumeration Reagent
Sterile, 96-well plates
Non-sterile, 96-well plates
Instruction manual

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