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CellExpand™ Suspension Expansion Culture™ (SEC™) Reagents for Lympho-Hematopoietic Cells

CellExpand™
Suspension Expansion Culture™ (SEC™) Reagents for Lympho-Hematopoietic Cells
Uses of CellExpand™
Expansion of stem and progenitor lympho-hematopoietic cells in suspension cultures.
Used for 96-well, 24-well, 12-well or 6-well plate suspension cultures or in large culture flask volumes.
Allows easy and rapid high-content screening for both proliferation and differentiation processes.
High throughput flow cytometry.
Cell signaling studies.
DNA, RNA analysis and other molecular biology methods.
Mechanism of Action (MOA) studies.
Evaluate the effects of drugs, xeonbiotics or other agents on the differentiation/maturation process.
Gene transfection and targeting studies.
Benefits of using CellExpand™ Reagents
Available low serum or serum-free (human and primate only) formulations.
Used in all HALO®, HemoFLUOR™ and HemoLIGHT™ Assay Kits.
Available for 6 stem cell populations and 7 progenitor cell populations.
Setup as many replicates as necessary.
Allows easy, rapid and accurate dispensing using normal pipettes or liquid handlers.
CellExpansion™ Master Mixes allow for cell interactions thereby providing: (1) Shorter lag time to initiate proliferation; (2) More rapid culture completion; (3) Greater sensitivity.
Species
CellExpand™ Low Serum Reagents are available for the for the following species:

Human
Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)
Dog
Rat
Mouse
CellExpand Serum-Free is available for human and primate cells only.



Cell Populations detected using CellExpand™ Reagents (see Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails)
CellExpand™ Reagents are available for the following cell populations:

Stem Cells

HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L
HPP-SP 2: EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L
CFC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L
CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO
CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF
CFC-GEM 2: EPO, GM-CSF, IL-3, SCF

Progenitor Cells

BFU-E 1: EPO, IL-3, SCF
BFU-E 2: EPO (high dose)
GM-CFC 1: GM-CSF, IL-3, SCF
GM-CFC 3: GM-CSF
Mk-CFC 1: TPO, IL-3, SCF
T-CFC: IL-2 (also requires addition of co-stimulator)
B-CFC: IL-7


Use HemoGro™-LS or HemoGro™-HPSF for controls (no growth factors)


Use
For Research Use Only. Not for clinical diagnostic use


Related Reagents and Assays
HemoGro™ Medium
ColonyGro™ Methylcellulose Colony-Forming Cell Master Mixes
CAMEO™-4
CAMEO™-96
HALO®-96 Research
HALO®-96 PREP
HemoFLUOR™-96
HemoLIGHT™-96

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