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SISTEMAS DE MARCAÇÃO E DETECÇÃO PARA IHC/WB/IP/FCM

Since 1976, Vector Laboratories has been at the forefront of developing innovative detection reagents. The VECTASTAIN® ABC systems established us as the leader in immunohistochemistry. Because of our commitment to developing novel technologies and products of consistent quality, Vector Laboratories remains a trusted name in this field. Our products are well regarded for their high sensitivity, low background, reliability, reproducibility, and value pricing. Many different detection systems are available from us to accommodate the wide range of experimental priorities.
VECTASTAIN® ABC Systems. With more than 40,000 citations to its credit, the VECTASTAIN® ABC kit remains widely popular. Based on the versatile biotin/avidin interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry laboratory.

ImmPRESS™ Peroxidase Polymer Reagents. The highly sensitive, one-step, ready-to-use, non-biotin, ImmPRESS™ Polymer Detection reagents are the result of novel conjugation methods and micropolymer chemistry developed at Vector Laboratories. Peroxidase micropolymers allow excellent resolution and crisp, strong staining of antigens, especially nuclear and membrane antigens. ImmPRESS™ reagents avoid the steric problems of other polymer-based reagents, thus providing superior results. These reagents are ideal for multiple antigen labeling because of the simplified one-step protocol.

Vector® M.O.M.™ (Mouse on Mouse) Immunodetection System. The specific detection of mouse primary antibodies on mouse tissue is difficult due to the presence of endogenous mouse immunoglobulins. A conventional anti-mouse detection system cannot distinguish between the mouse primary antibody and the endogenous mouse immunoglobulins leading to high background. The Vector® M.O.M.™ system is designed for the localization of mouse primary antibodies on mouse tissue. This system uses a VECTASTAIN®ABC staining method but contains a proprietary Mouse Ig Blocking Reagent and a specially modified anti-mouse Ig secondary antibody. Tedious calculations and primary antibody pre-binding steps are not required. Excellent staining results for a once difficult application have now become routine with the Vector® M.O.M.™ system.

Proprietary Enzyme Substrates. Along with the traditional chromogenic substrates Vector Laboratories also offers a wide variety of proprietary enzyme substrates. These proprietary substrates provide novel color choices essential for staining antigens in pigmented tissues and melanomas as well as for localizing multiple antigens in the same tissue section. Several substrates can be effectively viewed under darkfield or electron microscopy. Many of our substrates have unique spectral profiles that may be useful in the colocalization of antigens using spectral imaging systems.

Multiple Antigen Labeling. Accurate, reliable localization of two or more antigens on the same tissue section is a powerful research tool that can be applied in standard laboratory settings. The high specificity and low background of our reagents and proprietary enzyme substrates provide optimal sensitivities coupled with exquisite color combinations. Many of our substrates allow colocalization of antigens using spectral imaging.

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